Thermal Resistance of Proteolytic Enzymes Produced by Psychrotrophic Bacteria Isolated from Buffalo Milk

Proteolytic enzymes from extremely halophilic bacteria.

Halobacterium strains produce a truly extracellular proteinase which degrades gelatine and casein. It has a pH optimum of about 8 and depends upon divalent cations and a high concentration of NaCl or KC1 for activity and stability. Proteolytic enzymes were also found in cell homogenates obtained by ultra sonic treatment. A caseinolytic enzyme, probably different from the extracellular one, is a...

The Proteolytic Enzymes of Bacteria

A great deal of work has been done on the proteolytic enzymes of bacteria, especially those enzymes which are secreted into the medium by the living cell or as the result of its autolysis. The proteolytic enzymes of the cells themselves, however, have been less studied. Studies on bacterial peptidases in which substrates of known formulae (such as synthetic peptides) have been used, are few and...

Incidence of psychrotrophic lipolytic bacteria in cow’s raw milk

The contamination of bulk samples of cow’s raw milk (n = 491) by psychrotrophic lipolytic bacteria (PLiBC), total count of psychrotrophic bacteria (PBC) and mesophilic bacteria (TBC) was monitored for two years on eight dairy farms and the correlations among these groups of bacteria were analysed. An increase in TBC, PBC and PLiBC and in the values of free fatty acids (FFA) was tested experimen...

Characterisation of Antibiotic-resistant Psychrotrophic Bacteria in Raw Milk

Extracellular proteolytic enzymes produced by human pathogenic vibrio species

Bacteria in the genus Vibrio produce extracellular proteolytic enzymes to obtain nutrients via digestion of various protein substrates. However, the enzymes secreted by human pathogenic species have been documented to modulate the bacterial virulence. Several species including Vibrio cholerae and V. vulnificus are known to produce thermolysin-like metalloproteases termed vibriolysin. The vibrio...